A Perfect Day for Bananafish :: Perfect Day Bananafish

A Perfect Day for Bananafish Picture walking into a hotel room and finding a man dead on a bed. Upon closer inspection it becomes obvious that he has supposedly taken his own life with the gun that lay beside him. In talking to his wife who was asleep on the bed next to him when this incident occurred, it is learned that he just walked in the door and shot himself late the previous night. Out of the many questions that could be asked from this story, I believe that it is probably extremely important to consider why the main character, Seymour Glass, decided to commit suicide. What I believe to be the reason for Seymour’s suicide has two basic components: the spiritual depravity of the world around him, and his struggle with his own spiritual shortcomings. The spiritual problem of the outside world is mostly a matter of material greed, especially in the west, and materialism. On the other hand, his own spiritual problem is more a matter of intellectual greed and true spiritualism. In addressing the suicide, the difference should be distinguished between the "See More Glass" that we see through little Sybil’s eyes, and the Seymour Glass that we see through the eyes of the adult world. Even though these two characters are in theory the same man, they are slightly different in some ways. You could also say that they are the same character in different stages of development. Whatever the case may be, the "reasons" for the suicide shift slightly in emphasis as the character changes. "A Perfect Day for Bananafish" attempts to symbolize that the bananas in See More Glass’s story represent all of the things which are taken in along the journey to adulthood. If pursued with too much zeal, these bananas can prevent spiritual development and lead to a greater materialistic development. See-More has realized that he cannot get rid of enough bananas to make any further spiritual progress in this life, so, rather than waste time, he commits suicide. This is slightly obvious when he is taking the elevator back up to his room on the night of the suicide. His fixation upon his feet, which do not resemble the childlike feet that he desires to have, and the woman in the elevator’s scorn towards Seymour’s accusing her of staring at his feet, drive him to dislike the adult world even more.

Lab Results Fermenter

I. OBJECTIVES: To determine the amount of anti-microbial peptide production by Staphylococcus warneri under various conditions when 2L and 10L Fermented. To Test the effects of one uncontrolled parameters sush as pH, Temperature or dissolved Oxygen and compare findings. To produce anti-microbial activity from Staphylococcus warneri. II. INTRODUCTION: Staphylococcus warneri is a member of bacterial genus Staphylococcus, consisting of Gram-positive bacteria with spherical cells appearing in clusters. Colonies of S. warneri are usually tan, yellow and about 2-4mm in diameter after 48 hours incubation at 35 °C.It is commonly found as part of the skin flora on humans and animals. S. warneri rarely causes disease, but may occasionally cause infection in patients whose immune system is compromised. S. warneri is known to produce antimicrobial peptide activity in the form of Nisin. The optimum conditions for this to occur are pH 7. Nisin is a polycylic antibacterial peptide with 34 amino a cid residues used as a food preservative. It is produced by bacterium and which contains antimicrobial activity and which is known as a bacteriocin. Nisin has been found to have properties that can control spoilage caused by lactic acid bacteria.It is used in processed cheese, meats, beverages, etc. during production to extend shelf life by suppresing Gram-positive spoilage and pathogenic bacteria. In food it is common to use Nisin at levels depending on the food type regulatory approval. Nisin cannot be produced chemically therefore it has to be synthesised using fermentation. During fermentation various stages of growth occur and as a result different conditions can occur during this fermentation process, eg pH, most organisms produce acid as they grow and therefore in the Lag phase ( a period of adptation for the cells to their new environment, new enzymes are ynthesized) and in the lag phase can produce alkaline substances and therefore pH plays an important role in efficient fe rmentation. As acid is produced alkaline substance needs to be added to the process to maintain the optimum pH of 7 and likewise in the lag phase when alkaline substances are produced, acidic substance needs to be added to maintain the pH, temperature, and oxygen. III. MATERIALS AND METHODS:â€Å"As per manual. † IV. RESULTS: TABLE 1. 1 History Plot Vessel 1 – 2L NO Temperature control: TABLE 1. 2 History Plot Vessel 2 – 2L NO Air Flow: TABLE 1. History Plot Vessel 3 – 2L NO pH control: TABLE 1. 4 History Plot Vessel 4 – 2L Optimum conditions: TABLE 1. 5 History Plot Vessel 5 – 10L Optimum conditions: TABLE 1. 6 Fermentation conditions for each Vessels 1 – 5: Parameter Vessel Number Vessel 1 Vessel 2 Vessel 3 Vessel 4 Vessel 5 (10L) pH 7 7 No control 7 7 Agitation Speed (RPM) 150 150 150 150 150 Temp oC No Control 37 37 37 37 Airflow (L/min) 2 No air flow 2 2 2 TABLE 1. 7 Results for antimicrobial peptide activity in 2 L or 10 L ferme nters: Time (post inoculation) Vessel 1 Vessel 2 Vessel 3 Vessel 4 Vessel 5 3:00 (4. 5 hours) No activity No activity Neat Neat Neat; 1:2 14:00 (5. 5 hours) No activity Neat; 1:2 Neat; 1:2 Neat; 1:2 No results 15:00 (6. 5 hours) No activity Neat; 1:2 Neat Neat; 1:2 Neat; 1:2; 1:4;1:8 16:00 (7. 5 hours) No activity Neat Neat;1:2;1:4 Neat; 1:2 Neat; 1:2; 1:4;1:8 9:00 (24. 5 hours) No activity Neat Neat;1:2;1:4 Neat;1:2;1:4;1:8 Neat; 1:2; 1:4;1:8 V. DISCUSSION: In this practical, Fermentation is used to scale testing in laboratory. The fermenters in the Laboratory are based on a batch system, with feeds to control the pH and Oxygen levels and Temperature.All parameters are controlled using sensor probes in the vessels connected to a data logging software system. The vessels 4 and 5 are controls where the optimum environmental growth parameters for the strain are kept. To determine the results obtained in each vessels are as follows: Vessel 1: No antimicrobial peptide activity seen at a ny of the time intervals. This indicates that when temperature is not controlled the temperature can increase significantly. As shows in TABLE 1. 1 History Plot Vessel 1 – 2L NO Temperature control. Vessel 2: No antimicrobial activity seen at 13:00.However antimicrobial activity seen in both neat and 1:2 sample at 14:00 and 15:00. Antimicrobial activity seen in neat sample at 16:00 and 09:00. When air flow is not controlled the reduced air content reduces the rate of fermentation, As Oxygen is required for cell growth and when air is in reduced quantity this slows down rate of cell reproduction as shows in TABLE 1. 2 History Plot Vessel 2 – 2L NO Air Flow. Vessel 3: Antimicrobial activity seen in neat sample at all time intervals. Antimicrobial activity seen in 1:2 sample at 14:00, 16:00 and 09:00.Activity seen in 1:4 for the first time at 16:00 and 09:00. There is greater anti-microbial peptide activity with temperature and air controls which shows that the pH does no t have significant effects as the other two parameters. The fermentation was not affected to the same extent by pH as shown in TABLE 1. 3 History Plot Vessel 3 – 2L NO pH control. Vessel 4: Antimicrobial activity seen in neat sample at all time intervals. Activity seen in 1:2 sample at 14:00, 15:00, 16:00 and 09:00. For the first time see antimicrobial activity in 1:8 sample at 09:00.This shows the three uncontrolled vessels has greater anti-microbial peptide, where in fermentation took place on its fastest rate as all conditions are maintained at most favourable for the organism to grow and reproduced as shown in TABLE 1. 4 History Plot Vessel 4 – 2L Optimum conditions. Vessel 5: (In error no result recorded for 14:00 time interval) Antimicrobial activity seen in neat and 1:2 sample at all time intervals. Activity seen in 1:4 and 1:8 (for the first time) at 15:00, 16:00 and 09:00. The effects produce the highest level of anti-microbial peptide activity of all the syst em.The organism has greater supply of oxygen and nutrients and temperature and pH has a lesser effect due to the larger volume as shown in TABLE 1. 5 History Plot Vessel 5 – 10L Optimum conditions. VI. CONCLUSIONS: In this practical the results was successfully determined that Temperature is the most important parameter to control in relation to microbial growth. Therefore, if temperature was not controlled, NO amount of anti-microbial peptide activity produced by Staphylococcus warneri. While in Oxygen level and pH level if NOT controlled S. warneri will still grow and produced the anti-microbial peptide.

Analysis Of Don t Judge A Book - 950 Words

Sunil Shenoy Mr. Carmona; MYP 5 Honors English, Period 2 11 October 2015 Word Count: 937 Don’t Judge a Book by its Cover Today, in modern society we are commonly told to not judge others for their appearance but only for their personal features and characteristics. While equality is far from being accomplished, inner qualities are appreciated much more and the idea that only the appearance of a person matters has been diminished. However, through the major characters’ personalities and dialogue in both As You Like It by William Shakespeare and A Doll’s House by Henrik Ibsen, a central idea of false appearance symbolically and realistically unifies both plays. At the beginning of both plays, there is an obvious contrast in personality between Nora and Orlando. After learning that Orlando has beaten the famous wrestler Charles, the audience anticipates that he is a modest yet courageous character. While this achievement depicts him as gallant, his continuous love poems to Rosalind suggest a softer, more sensitive personality. â€Å"I am he that is so love-shaked. I pray you tell me your remedy,† he begs (3.2.329-330). This is continually demonstrated throughout the play, which therefore eliminates the previous notion of him. â€Å"I would not have my right Rosalind of this mind, for I protest her frown might kill me† (4.1.94-95). Orlando is clearly a more romantic and loving man than understood after the wrestling match, illustrating his true personality. In contrast, Nora isShow MoreRelatedAnalysis Of The Book Don t Judge A Boy 1168 Words   |  5 PagesI. SHORT ANSWER 1. The cover of the book uses the tagline â€Å"Don’t judge a boy by his face†, because the book is about a boy named Auggie who does not look like everybody else. When he goes to school for the first time, most of his other fifth grade peers decide to judge him, not become his friend, and try to avoid him. 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